Dentinal tubule occluding capability of nano-hydroxyapatite; The in-vitro evaluation

. 2018 Aug;81(8):843-854.

Authors

Serdar Baglar  ¹ , Umit Erdem  ² , Mustafa Dogan  ² , Mustafa Turkoz  ³

Affiliations

• ¹ Department of Restorative Dentistry, Faculty of Dentistry, Kirikkale University, Kirikkale, 71450, Turkey.

• ² Scientific and Technological Research Application and Research Center, Kirikkale University, Kirikkale, 71450, Turkey.

• ³ Faculty of Engineering, Department of Electric and Electronics Engineering, Karabük University, Karabuk, 78050, Turkey.

  Abstract

  In this in-vitro study, the effectiveness of experimental pure nano-hydroxyapatite (nHAP) and 1%, 2%, and 3% F¯ doped nano-HAp on dentine tubule occlusion was investigated. And also, the cytotoxicity of materials used in the experiment was evaluated. Nano-HAp types were synthesized by the precipitation method. Forty dentin specimens were randomly divided into five groups of; 1-no treatment (control), 2-specimens treated with 10% pure nano-HAp and 3, 4, 5 specimens treated with 1%, 2%, and 3% F^- doped 10% nano-HAp, respectively. To evaluate the effectiveness of the materials used; pH, FTIR, and scanning electron microscopy evaluations were performed before and after degredation in simulated body fluid. To determine cytotoxicity of the materials, MTT assay was performed. Statistical evaluations were performed with F and t tests. All of the nano-HAp materials used in this study built up an effective covering layer on the dentin surfaces even with plugs in tubules. It was found that this layer had also a resistance to degradation. None of the evaluated nano-HAp types were have toxicity. Fluoride doping showed a positive effect on physical and chemical stability until a critical value of 1% F^- . The all evaluated nano-HAp types may be effectively used in dentin hypersensitivity treatment. The formed nano-HAp layers were seem to resistant to hydrolic deletion. The pure and 1% F^- doped nano-HAp showed the highest biocompatibility thus it was assessed that pure and 1% F^- doped materials may be used as an active ingredient in dentin hypersensitivity agents.

  Keywords: cytotoxicity; degredation; dentine hypersensitivity; dentine tubule occlusion; fluoride; hydroxyapatite.