본문
Dent Mater
2020 Jul;36(7):e207-e216.
doi: 10.1016/j.dental.2020.04.005. Epub 2020 May 13.
The effect of
disaggregated nano-hydroxyapatite on oral biofilm in vitro
분해 된 나노 하이드록시아파타이트가
구강 세균막(치면세균막)에 미치는 효과
Weidan Luo 1 , Yannan Huang 1 , Xuedong Zhou 1 , Qi Han 2 , Xian Peng 3 , Biao Ren 3 , Jiyao Li 1 , Mingyun Li 4 , Lei Cheng 5
Affiliations Expand
Affiliations
1 State Key Laboratory of Oral Diseases, West China
Hospital of Stomatology, National Clinical Research Centre for Oral Diseases,
Sichuan University, Chengdu 610064, China; Department of Operative Dentistry
and Endodontics, West China Hospital of Stomatology, Sichuan University,
Chengdu, China. 2 State Key Laboratory of Oral Diseases, West China
Hospital of Stomatology, National Clinical Research Centre for Oral Diseases,
Sichuan University, Chengdu 610064, China; Department of Oral Pathology, West China
Hospital of Stomatology, Sichuan University, Chengdu, China. 3 State Key Laboratory of Oral Diseases, West China
Hospital of Stomatology, National Clinical Research Centre for Oral Diseases,
Sichuan University, Chengdu 610064, China. 4 State Key Laboratory of Oral Diseases, West China
Hospital of Stomatology, National Clinical Research Centre for Oral Diseases,
Sichuan University, Chengdu 610064, China. Electronic address:
limingyun@scu.edu.cn. 5 State Key Laboratory of Oral Diseases, West China
Hospital of Stomatology, National Clinical Research Centre for Oral Diseases,
Sichuan University, Chengdu 610064, China; Department of Operative Dentistry
and Endodontics, West China Hospital of Stomatology, Sichuan University,
Chengdu, China. Electronic address: chenglei@scu.edu.cn. Abstract Objective: Agglomeration is a common problem facing the
preparation and application of nanomaterials, and whether nano-hydroxyapatite
(nano HA) can modulate oral microecology left to be unclear. In this study,
nano HA was disaggregated by sodium hexametaphosphate (SHMP) and ultrasonic
cavitation to observe whether agglomeration would affect its effect on oral
bacterial biofilm. 응집(덩어리는) 나노물질
적용시 흔한 방해요소중 하나이며 나노하이드록시아파타이트가 구강 미세 생태학을 바꾸는지도 명확치 않다. 본
연구에서는 나노 하이드록시아파타이트는 응집이 구강내 세균막에 영향을 주는지를 관찰하기 위해 헥사메타딘나트륨과 초음파 케비테이션으로 분해 하였다. Methods: Dynamic light scattering (DLS) and scanning
electronic microscope (SEM) were used to observe the treatment solutions.
Single-species biofilms and multi-species biofilms were treated with 10% nano
HA, 10% disaggregated nano HA, 10% micro hydroxyapatite (micro HA) and
deionized water (DDW) for 30min and analyzed via MTT assay, lactic acid
measurement, SEM and confocal laser scanning microscope (CLSM). Real-time
polymerase chain reaction was performed to analyze the biofilm composition. Results: Ultrasonic cavitation combined with SHMP could
significantly reduce the degree of agglomeration of nano HA. Disaggregated nano
HA could inhibit bacterial growth and reduce the ability of bacterial biofilm
to produce lactic acid and extracellular polysaccharides. There was no
significant difference on composition of multi-species biofilms between nano HA
and disaggregated nano HA. 핵사메타인산나트륨을 이용한 초음파 케비테이션이 나노 하이드록시아파타이트의 응집 정도를 크게 낮추었다. 분해 된 나노 하이드록시아파타이트는 구강네균의 성장을 억제할 수 있고 젖산과 세포밖 다당류를 만드는 세균막의 능력을
억제 할 수 있다. Significance: The disaggregated nano-hydroxyapatite could inhibit
the metabolism and acid production of oral bacterial biofilm, but did not
significantly affect the composition of multi-species biofilms. 분해 된 나노 하이드록시아파타이트는 구강내 세균막의 산 배출과 대사작용을 억제 할 수 있다. Keywords: Agglomeration; Biofilms; Dental caries; Dispersant;
Nano-hydroxyapatite. BMC Oral Health 2019 May 28;19(1):92. doi: 10.1186/s12903-019-0785-6. Effect of
hydroxyapatite nanoparticles on enamel remineralization and estimation of
fissure sealant bond strength to remineralized tooth surfaces: an in vitro
study 하이드록시아파타이트 나노입자가 에나멜
재광와 재광화된 치아표면의 실란트의 접착력에 미치는 효과 Mahtab Memarpour 1 , Fereshteh
Shafiei 2 , Azade Rafiee 3 , Mina Soltani 1 , Mohammad Hossein
Dashti 4 Affiliations Expand Affiliations
1 Oral and Dental Disease Research Center, Department
of Pediatric Dentistry, School of Dentistry, Shiraz University of Medical
Sciences, Shiraz, Iran. 2 Oral and Dental Disease Research Center, Department
of Operative Dentistry, School of Dentistry, Shiraz University of Medical
Sciences, Shiraz, Iran. 3 Oral and Dental Disease Research Center, Department
of Pediatric Dentistry, School of Dentistry, Shiraz University of Medical
Sciences, Shiraz, Iran. arafiee@sums.ac.ir. 4 Department of Restorative Sciences and Biomaterials,
Henry M. Goldman School of Dental Medicine, Boston University, Boston, MA, USA. Abstract Background: The management of noncavitated caries lesions before
sealant therapy is a clinical challenge when the tooth needs sealant
application. Sealing noncavitated carious lesions in pits and fissures may lead
to failure of the fissure sealant (FS) due to incomplete sealing. Therefore the
use of remineralizing agents such as nanoparticles has been suggested. This
study investigated the ability of hydroxyapatite nanoparticles (nano-HA) to
remineralize enamel, and their effect on sealant microleakage and shear bond
strength (SBS). 실란트 치료 전의 활성화 되지 않는 초기 충치의 유지관리는 실란트 치료적용시 매우 중요한 이슈이다. 초기충치를 실란트로 봉쇄하는 것은 불완전한 밀페력으로 인해 실란트 실패로 이어질 수 있다. 따라서 나노 하이드록시아파타이트와 같은 재광화 물질을 사용이 요구 되는 것이다. 본
연구는 나노 하이드록시아파타이트의 에나멜 재광화 능력과 실란트의 미세누출과 접착력에 미치는 영향을 알아보기 위한 것이다. Methods: A total of 192 third molars were demineralized and
pretreated with two concentrations of nano-HA with and without sodium
hexametaphosphate (SHMP), followed by phosphoric acid etching and resin FS
application. The study groups were 1) etching + FS, 2) etching + nano-HA 0.15%
+ FS, 3) etching + nano-HA 0.03% + FS, 4) etching + mixture of nano-HA 0.15%
and SHMP 0.05% + FS, 5) etching + mixture of nano-HA 0.03% + SHMP 0.01% + FS.
The laboratory tests included microleakage in 50 teeth, scanning electron
microscopy (SEM) evaluation in 10 samples, and SBS in 100 samples. Enamel
remineralization changes were evaluated in 32 teeth with energy-dispersive
X-ray spectroscopy (EDS) and field emission scanning electron microscope
(FESEM). Results: Nano-HA enhanced the SBS to remineralized enamel in a
large percentage of nanoparticles. Mean SBS in group 2 was significantly
greater than in groups 1, 3 and 4 (all P < 0.05). SBS was related to nano-HA
concentration: nano-HA 0.15% yielded greater SBS (16.8 ± 2.7) than the 0.03%
concentration (14.2 ± 2.1). However, its effect on microleakage was not
significant. Nano-HA with or without SHMP led to enhanced enamel
remineralization; however, the Calcium (Ca)/Phosphate (P) weight percent values
did not differ significantly between the groups (P > 0.05). SEM images
showed that SHMP did not affect sealant penetration into the deeper parts of
fissures. FESEM images showed that adding SHMP led to increased nanoparticle
dispersal on the tooth surface and less cluster formation. 나노 하이드록시아파ㅏ이트는 에나멜의 재광화을 증진 시킨다. 헥사메타인산나트륨의 존재여부와 관계없이 에나멜 재광화를 증진시킨다. Conclusions: The ultraconservative approach (combining nano-HA
0.15% and SHMP) and FS may be considered a minimal intervention in dentistry to
seal demineralized enamel pits and fissures. 탈회된 에나멜의 실란트 봉쇄는 최초침습 개념에 근거해 매우 보수적인 접근(나노 하이드록시아파타이트 0.15% 와 핵사메타인산나트륨)이 필요하다. Keywords: Energy-dispersive X-ray spectroscopy;
Nano-hydroxyapatite; Pit and fissure sealant; Remineralization. J Dent 2011 Sep;39(9):629-35. doi: 10.1016/j.jdent.2011.07.001. Epub 2011 Jul 7. The addition of
nano-sized hydroxyapatite to a sports drink to inhibit dental erosion: in vitro
study using bovine enamel 치아부식방지를 위한 스포츠 음료에 나노
크기 하이드록시아파타이트 첨가 J H Min 1 , H K Kwon, B I Kim
Affiliations Expand Affiliation
1 Department of Preventive Dentistry and Public Oral
Health, Research Center for Orofacial Hard Tissue Regeneration, College of
Dentistry, Yonsei University, Seoul, Republic of Korea. Abstract Objectives: This study examined the dental erosion and
demineralization potential of a sports drink containing nano-sized
hydroxyapatite (nano-HA) as an additive. 본 연구는 나노 입자 하이드록시아파타이트를
첨가제로 넣은 스포츠 음료의 치아부식과 재광화 효과를 검증하기 위한 것이다. Methods: The experimental solutions were Powerade (PA) alone
and PA with 0.05%, 0.10%, and 0.25% nano-HA. The pH, titratable acidity, and
calcium and phosphate content of each solution were analysed, and the degree of
saturation with respect to the dental enamel (DS(En)) was obtained. Twelve
sound bovine enamel specimens for each group were treated in accordance with
the pH-cycling schedule which had 60min treatment with experimental solution
per day for 7 days. The erosion potential was determined from the changes in
surface micro hardness (SMH), the depths of erosion and demineralized layer
using confocal laser scanning microscopy (CLSM), and the morphological changes
to the tooth surface were examined with scanning electron microscopy (SEM)
after pH-cycling. Results: pH and DS(En) increased with increasing nano-HA
concentration in the drinks, whereas the titratable acidity decreased. There
were significant differences in the SMH between the PA alone and >0.10%
nano-HA groups (p<0.001). Although the PA alone group showed a pronounced
erosion depth, CLSM showed no erosion depth in 0.25% nano-HA group. SEM showed
an intact surface with increasing nano-HA concentration in the drinks. In conclusion, dental erosion was
effectively prevented with increase of adding concentration of nano-HA, and a
sports drink containing 0.25% nano-HA might prevent dental erosion. 결론적으로, 치아부식은 나노 하이드록시아파타이트의 증가에 따라
효과적으로 예방 되며, 스포츠 음료에 함유된 0.25% 나노 하이드록시아파타이트는
치아부식을 예방할 수 있다.
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