| subject | 상아질의 탈회와 재광화: 나노하이드록시아파타이트 nHAP의 재광화효과 |
|---|---|
| writer | 관리자 |
| date | 19-05-20 12:23 |
| hit | 1,420 |
관련링크본문Dent Mater. 2019 Apr;35(4):617-626. doi: 10.1016/j.dental.2019.02.007. Epub 2019 Feb 23. Monitoring demineralization and remineralization of human dentin by characterization of its structure with resonance-enhanced AFM-IR chemical mapping, nanoindentation, and SEM.AbstractOBJECTIVE:This research aimed at monitoring demineralization and remineralization of dentin and its collagen matrix at the nanoscale by amorphous, microcrystalline, and in situ formed hydroxyapatite. METHODS:The concurrent use of the resonance-enhanced atomic force microscopy coupled with infrared probe (AFM-IR) chemical mapping, nano-indentation, and scanning electron microscopy (SEM) provides a detailed insight into the structure of human dentin, as well as to the processes of its partial demineralization and remineralization. RESULTS:The resonance-enhanced AFM-IR chemical mapping of dentin has shown to be a useful method to follow distribution of its collagen and hydroxyapatite components at the micro- and nanoscale levels, especially in conjunction with SEM imaging and nanoindentation. Dentin with a higher extent of natural dentin tubule occlusion tends to be harder and less elastic. The relative affinity of the collagen and hydroxyapatite components of dentin toward hydroxyapatite depends on its type (amorphous, microcrystalline, or formed in-situ). The gel mineralization technique allows for an even and controlled growth of hydroxyapatite guided by the completely demineralized collagen matrix of dentin. SIGNIFICANCE:The observed trends of the affinity of collagen toward different forms of hydroxyapatite helps develop new remineralizing formulations. The employed methods of characterization may provide an insight to the natural processes of bone mineralization guided by its both hydroxyapatite and protein constituents.
J Conserv Dent. 2018 Nov-Dec;21(6):681-690. doi: 10.4103/JCD.JCD_31_18. Evaluation of remineralization potential and mechanical properties of pit and fissure sealants fortified with nano-hydroxyapatite and nano-amorphous calcium phosphate fillers: An in vitro study.AbstractObjectives:The aim of this study was: (i) to formulate pit and fissure sealants (PFS) containing nano-hydroxyapatite (nHAP) filler; nHAP filler and silica co-filler; nHAP and nano-Amorphous Calcium Phosphate (nACP) co-filler, (ii) to evaluate physical properties; degree of conversion (DOC), curing depth (CD) and mechanical properties; microshear bond strength (MBS) of fortified PFS, and (iii) to assess remineralization potential and release of Ca2= and PO4 ions from newly synthesized sealants. Materials and Methods:Four PFS were prepared using monomers with mixture of 35.5 wt % BisGMA, 35.5 wt % triethylene glycol dimethacrylate and 28 wt % hydroxyethyl methacrylate. Bioactive nanofillers (nHAP and n-ACP) were added in various concentrations (0%-30%). Three commercial sealants were used as follows: unfilled (Clinpro; 3M ESPE), Fluoride releasing (Delton FS plus, Dentsply), ACP filled (Aegis, Bosworth). The samples (n = 35.5/gp) were tested for MBS, DOC, and CD. Remineralization potential was assessed by scanning electron microscopy (SEM). The concentrations of Ca2= and PO4 released from the sealant specimens were analyzed with Ultraviolet-visible Spectrophotometer. Data obtained was statistically analyzed (one-way analysis of variance, Tukey's test, P < 0.05). Results:10% hydroxyapatite (HAP) =20% ACP sealant showed significantly higher DOC. A remineralized region on the surface between fissure sealant and tooth enamel was observed by SEM in all three HAP filled bioactive sealants. Decreasing the solution pH significantly increased ion release from sealant filled with 10% nHAP = 20% nACP (P ≤ 0.001). Conclusion:Results suggested that admixture of nHAP and nACP to PFS showed remineralizing capability, without declining their mechanical and physical properties. |
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https://www.ncbi.nlm.nih.gov/pubmed/30808558
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